5th International Conference on Analysis of Microbial Cells

Sat. 05

Sun. 06

Mon. 07

Tue. 08

07:00

08:00

09:00

10:00

11:00

12:00

13:00

14:00

15:00

16:00

17:00

18:00

19:00

20:00

21:00

22:00

Opening Session

14:00 - 14:15 (15min)

Opening Session

Lectures

Jeff Cole & Gérald Grégori

Biotechnology

14:15 - 16:10 (1h55)

Biotechnology

Lectures

Gerhard Nebe-von-Caron

› Multi-parameter flow cytometry as a tool to monitor microbial oil production towards biofuel - Invited Speaker : Teresa Lopes da Silva, National Laboratory of Energy and Geology - Bioenergy Unity 14:15-14:50 (35min)

› Monitoring microbial population dynamics in a labscale biogas reactor using flow cytometry - Christin Koch, Helmholtz Centre for Environmental Research 14:50-15:10 (20min)

› Biosensor based analysis of microbial production strains - Frunzke Julia, Institute of Bio- and Geosciences, IBG1: Biotechnology 15:10-15:30 (20min)

› Single cell-scale metabolite sensor-based system for rapid isolation of bacterial metabolite producers - Eggeling Lothar, Research Centre Jülich GmbH, IBG-1: Systems Microbiology 15:30-15:50 (20min)

› The Mechanism of Hydrocarbon Uptake by Pseudomonas fluorescens in Lubricating Oil Biodegradation - Ni'matuzahroh Ni'matuzahroh, Microbiology Laboratory, Biology Department, Science and Technology Faculty, Airlangga University, Indonesia 15:50-16:10 (20min)

Coffee break

16:10 - 16:40 (30min)

Coffee break

Lectures

Biotechnology

16:40 - 18:00 (1h20)

Biotechnology

Lectures

Gerhard Nebe-von-Caron

› Changes in viability and physiology of starter culture Lactococcus lactis subsp. cremoris AM2 during Cheddar cheese manufacture as monitored by flow cytometry and cell sorting. - Imelda Doolan, University of Limerick 16:40-17:00 (20min)

› The application of flow cytometry towards understanding the effects of processing treatments on food borne pathogens - Deirdre Kennedy, University of Limerick 17:00-17:20 (20min)

› Phenotypic characterization of Aspergillus niger strains in submerged cultivation and at the single cell level. - Mhairi McIntyre Workman, Center for Microbial Biotechnology 17:20-17:40 (20min)

› Omega-3 fatty acids act on Bifidobacterium animalis subsp. lactis survival during storage of organic fermented milks - Ana Carolina FLORENCE, AgroParisTech - INRA CBAI LGMPA, FCF - USP - Faculdade de Ciencias Farmacêuticas 17:40-18:00 (20min)

Refreshment / Demos / Posters

18:00 - 18:30 (30min)

Refreshment / Demos / Posters

Posters

Round table - Single cell analysis : State of the Art and New Trends

18:30 - 19:30 (1h)

Round table - Single cell analysis : State of the Art and New Trends

Lectures

Dinner

19:30 - 21:00 (1h30)

Dinner

Demos / Posters

21:00 - 22:00 (1h)

Demos / Posters

Presentation of the Posters - Demos of the Instruments

Breakfast

7:30 - 8:30 (1h)

Breakfast

Cell Biology

8:30 - 9:45 (1h15)

Cell Biology

Lectures

Kenji Yasuda

› Algebraic and geometric understanding of cells, epigenetic inheritance of phenotypes between generations using on-chip single cell cultivation system - Invited Speaker : Kenji Yasuda, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University 08:30-09:05 (35min)

› Bacterial persistence based on epigenetically correlated stochastic gene expression - Wakamoto Yuichi, Research Center for Complex Systems Biology 09:05-09:25 (20min)

› Single-growing units phenotypic heterogeneity in Streptococcus thermophilus DSM20617 - Mora Diego, Dipartimento di Scienze e Tecnologie Alimentari e Microbiologiche 09:25-09:45 (20min)

Coffee break

9:45 - 10:15 (30min)

Coffee break

From data analysis to mathematical modelling

10:15 - 11:30 (1h15)

From data analysis to mathematical modelling

Lectures

Hazel Davey

› Flow cytometry data analysis using Bioconductor. - Invited Speaker : Nolwenn Le Meur, Ecole des Hautes Etudes en Santé Publique 10:15-10:50 (35min)

› Flow, Nets and GPs - Hazel Davey, Institute of Biological, Environmental and Rural Sciences 10:50-11:10 (20min)

› Functional Data Analysis and Classification of Phytoplankton Based on Autonomous Flow Cytometer Data - Malkassian Anthony, Laboratoire de Microbiologie, de Géochimie et d'Ecologie Marines 11:10-11:30 (20min)

Lunch

11:30 - 13:30 (2h)

Lunch

Single cell approaches to cytomics, transcriptomics and proteomics

13:30 - 15:05 (1h35)

Single cell approaches to cytomics, transcriptomics and proteomics

Susann Müller

› Bridging the Gap - Cytomics meets Proteomics - Invited Speaker : Frank Schmidt, Ernst-Moritz-Arndt-University of Greifswald - Interfaculty Institute for Genetics and Functional Genomics 13:30-14:05 (35min)

› FUNCTIONAL SINGLE CELL ANALYTICS – STUDYING DNA PATTERNS AND DYNAMICS OF POLYPHOSPHATE ACCUMULATING BACTERIA IN WASTE WATER COMMUNITIES - Susanne Gunther, Department of Environmental Microbiology - Susann Müller, Department of Environmental Microbiology 14:05-14:25 (20min)

› Flow cytometric monitoring of a three-species mixed bacterial community relevant for Cystic Fibrosis - Rüger Marc, Otto von Guericke University, Bioprocess Engineering, Magdeburg, Germany 14:25-14:45 (20min)

› Physical interactions between Desulfovibrio vulgaris Hildenborough and Clostridium acetobutylicum are required to induce metabolic changes in the consortia - Marie Thérèse Giudici-Orticoni, Bioenergetic and protein engineering laboratory 14:45-15:05 (20min)

Human Health

15:05 - 16:20 (1h15)

Human Health

Lectures

Roy Bongaerts

› No more plating? Fast methods for microbial parameters and for screening of pathogens in drinking water analysis using flow cytometry - Invited Speaker : Thomas Egli, Swiss Federal Institute of Aquatic Science and Technology 15:05-15:40 (35min)

› Identification of Klebsiella pneumoniae's strains isolated from « urine » as a human pathological product and evaluation of their antibiotic resistance. - karima BENSALEM, Laboratory of Biofilms and Biocontaminations of Materials 15:40-16:00 (20min)

› Use of flow cytometry and FACS to isolate antibiotic resistant Salmonella enterica sv. Typhimurium following exposure to biocides - Tim Overton, Bioengineering, School of Chemical Engineering, University of Birmingham 16:00-16:20 (20min)

Coffee break

16:20 - 17:00 (40min)

Coffee break

Lectures

Presentation PARTEC

17:00 - 17:20 (20min)

Presentation PARTEC

Presentation by PARTEC

Presentation ACCURI-BD

17:20 - 17:40 (20min)

Presentation ACCURI-BD

Presentation by ACCURI-BD

Presentation CYTOBUOY

17:40 - 18:00 (20min)

Presentation CYTOBUOY

Presentation by CYTOBUOY

Presentation APOGEE

18:00 - 18:20 (20min)

Presentation APOGEE

Presentation by APOGEE

Presentation QIOPtics

18:20 - 18:40 (20min)

Presentation QIOPtics

Presentation by QIOPtics

Refreshment / Demos / Posters

18:40 - 19:30 (50min)

Refreshment / Demos / Posters

Posters

Dinner

19:30 - 21:00 (1h30)

Dinner

Demos / Posters

21:00 - 22:00 (1h)

Demos / Posters

Presentation of the Posters - Demos of the Instruments

Breakfast

7:30 - 8:30 (1h)

Breakfast

Natural Environment

8:30 - 9:45 (1h15)

Natural Environment

Lectures

Gérald Grégori

› Microautoradiography combined to fluorescence in situ hybridization as a way to interrogate single cells about their phylogeny and activity: a review of its use in marine microbial ecology, of its advantages and limitations, and some forecast - Invited Speaker : Josep M Gasol, Institut de Ciències del Mar 08:30-09:05 (35min)

› High-throughput analysis of soil eukaryotic unicellular microorganisms: from cytometry to pyrosequencing - Guillaume Lentendu, Helmholtz Centre for Environmental Research, Molecular Evolution & Animal Systematics, University of Leipzig, Plant Physiology, University of Leipzig 09:05-09:25 (20min)

› Calculating the carbon content of a drop in the ocean: alternatives to chlorophyll a in phytoplankton biomass estimation. - Katy R. Owen, Laboratory for Global Marine and Atmospheric Chemistry, Centre for Environment, Fisheries and Aquaculture Science 09:25-09:45 (20min)

Coffee break

9:45 - 10:15 (30min)

Coffee break

Natural Environment

10:15 - 11:35 (1h20)

Natural Environment

Lectures

Gérald Grégori

› Differential growth and mortality rates of Prochlorococcus, Synechococcus and picoeukaryotes across subarctic and subtropical waters - Francois Ribalet, School of Oceanography, University of Washington 10:15-10:35 (20min)

› Phytoplankton monitoring: pulse shape recording flow cytometry for an in situ high resolution observing approach - Melilotus Thyssen, Laboratoire d'Océanologie et de Géosciences (LOG, Wimereux) 10:35-10:55 (20min)

› Phytoplankton dynamics in the temperate coastal waters assessed by flow cytometry: the DYMAPHY project - Luis Felipe Artigas, Laboratoire d'Océanologie et Géosciences - Université du Littoral Côte d'Opale 10:55-11:15 (20min)

› Light dependence of phosphorus uptake by microorganisms in the North and South Pacific subtropical gyres - Solange Duhamel, University of Hawaii - Center for Microbial Oceanography: Research and Education 11:15-11:35 (20min)

Lunch

11:35 - 13:30 (1h55)

Lunch

New instrumental developments and techniques

13:30 - 16:05 (2h35)

New instrumental developments and techniques

Lectures

J. Paul Robinson

› High-throughput multiparametric flow cytometry – how does the new hardware impact our interaction with data? - Invited Speaker : Bartek Rajwa, Bindley Bioscience Center, Purdue University 13:30-14:05 (35min)

› SeaFlow: A novel underway cytometer for continuous observations of phytoplankton in the ocean - Jarred Swalwell, School of Oceanography, University of Washington 14:05-14:25 (20min)

› FluidFM: A novel nanofluidics instrument for single cell applications - Julia A. Vorholt, Institute of Microbiology and Institute of Biomechanical Engineering 14:25-14:45 (20min)

› Rapid Biomarker Profiling Of Escherichia coli Utilising MALDI-TOF Mass Spectrometry and a Multivariate Pattern Recognition Approach - Remi Momo, School of Chemical Engineering and Advanced Materials 14:45-15:05 (20min)

› A microfluidic cytometer exploiting a spatially modulated fluorescence signal to simultaneously measure fluorescent intensity and size on a single cell level - Sommer Christian, Institut für Mikrotechnik Mainz - Baßler Michael, Institut für Mikrotechnik Mainz 15:05-15:25 (20min)

› Hyperspectral Cytometry at the Single-Cell Level Using a 32-Channel Photodetector : preliminary results on bacteria - Gérald Grégori, Laboratoire de Microbiologie, Géochimie et Ecologie Marines 15:25-15:45 (20min)

› Detection of enzyme-labeled fluorescence phosphate (ELF-P) hydrolysis in the phytoplankton communities of North Pacific Subtropical Gyre - Mathias Girault, Tokyo University Marine Science and Technology, Dept. Ocean Sciences 15:45-16:05 (20min)

Coffee break

16:05 - 16:35 (30min)

Coffee break

New instrumental developments and techniques

16:35 - 17:15 (40min)

New instrumental developments and techniques

Lectures

J. Paul Robinson

› Monitoring plasmid stability in agar plate cultures of recombinant protein producing Escherchia coli expressing using GFP-based flow cytometry. - Christopher Wyre, School of Chemical Engineering, University of Birmingham 16:35-16:55 (20min)

› Single-cell fingerprint dynamics of antibody fragment secreting Bacillus megaterium cells - Florian David, Institute of Biochemical Engineering, Technische Universität Braunschweig 16:55-17:15 (20min)

Closing Session

17:15 - 18:00 (45min)

Closing Session

Lectures

Jeff Cole & Gérald Grégori

Free time

18:00 - 20:00 (2h)

Free time

Closing Gala

20:00 - 22:00 (2h)

Closing Gala

Breakfast

7:30 - 8:30 (1h)

Breakfast

Departures

8:30 - 10:00 (1h30)

Departures

Departure of the participants